ACKERMANN W W, ISHIDA N
J Exp Med. 1956 Oct 1;104(4):501-15. doi: 10.1084/jem.104.4.501.
The rate has been determined at which the initial stable complex is formed between cells of the chorioallantoic membrane and the PR8 strain of type A influenza virus. Characterization of the complex revealed the following properties: (a) stability to dilution and to simple washing with buffered saline, (b) insensitivity to the action of receptor-destroying enzyme (obtained from cholera vibrio), (c) sensitivity to the inhibitory effect of anti-viral immune serum, (d) formation of the complex at low temperatures, 3 degrees C., (e) accompanied by a loss of detectable viral infectivity from the reaction mixture, (f) stability at 3 degrees C. but transformation at 37 degrees C. to a state which is resistant to the inhibitory action of a specific anti-viral serum. The rate of this latter transformation has been determined. The infectious process in more than 50 per cent of the cells can be influenced by immune serum 2 hours after the completion of the initial reaction with virus.
已确定绒膜尿囊膜细胞与甲型流感病毒PR8株之间形成初始稳定复合物的速率。对该复合物的特性进行表征后发现了以下特性：(a) 对稀释和用缓冲盐水简单洗涤具有稳定性，(b) 对受体破坏酶（从霍乱弧菌获得）的作用不敏感，(c) 对抗病毒免疫血清的抑制作用敏感，(d) 在低温3℃下形成复合物，(e) 反应混合物中可检测到的病毒感染性丧失，(f) 在3℃下稳定，但在37℃下转变为对特异性抗病毒血清的抑制作用具有抗性的状态。已确定了后一种转变的速率。在与病毒的初始反应完成2小时后，超过50%的细胞中的感染过程可受免疫血清影响。
