Gallin E K, Green S W, Patchen M L
Department of Physiology, Armed Forces Radiobiology Research Institute, Bethesda, MD 20889.
Int J Immunopharmacol. 1992 Feb;14(2):173-83. doi: 10.1016/0192-0561(92)90028-j.
In order to compare both the actions of soluble glucan (glucan-F) and particulate glucan (glucan-P) on macrophages and the responsiveness of macrophages from C3H/HeJ and C3H/HeN mice to these immunomodulators, interleukin-1 (IL-1) levels, phagocytosis and superoxide production were monitored after an in vitro exposure to glucan-F or glucan-P. A 2 or 20 h exposure to either glucan preparation decreased the ability of both C3H/HeJ and C3H/HeN macrophages to ingest zymosan. In contrast, glucan-P, but not glucan-F, decreased (after a 20 h exposure) the uptake of both IgG opsonized erythrocytes and latex beads. Furthermore, glucan-P, but not glucan-F was as effective as zymosan (after a 1 h exposure) in inducing superoxide release by macrophages isolated from both C3H/HeN and C3H/HeJ mice. While the effects of glucan-P on PMA-induced superoxide release and IL-1 levels were similar in macrophages from C3H/HeJ and C3H/HeN mice, glucan-F was ineffective at enhancing PMA-induced superoxide release or increasing IL-1 levels in C3H/HeJ mice. Thus (1) the effects of glucan-P on phagocytosis of opsonized erythrocytes and latex beads are not mimicked by glucan-F and (2) while macrophages from C3H/HeJ mice respond normally (as compared with C3H/HeN macrophages) to glucan-P, they are hyporesponders to glucan-F. These findings indicate that the activation of macrophages by glucan-P involves different (or additional) pathways from those activated by glucan-F.
为了比较可溶性葡聚糖（葡聚糖 - F）和颗粒性葡聚糖（葡聚糖 - P）对巨噬细胞的作用以及C3H/HeJ和C3H/HeN小鼠巨噬细胞对这些免疫调节剂的反应性，在体外将细胞暴露于葡聚糖 - F或葡聚糖 - P后，监测白细胞介素 - 1（IL - 1）水平、吞噬作用和超氧化物产生。将细胞暴露于任一葡聚糖制剂2或20小时后，C3H/HeJ和C3H/HeN巨噬细胞摄取酵母聚糖的能力均下降。相比之下，葡聚糖 - P（暴露20小时后）而非葡聚糖 - F，降低了IgG调理红细胞和乳胶珠的摄取。此外，葡聚糖 - P而非葡聚糖 - F（暴露1小时后）在诱导从C3H/HeN和C3H/HeJ小鼠分离的巨噬细胞释放超氧化物方面与酵母聚糖效果相当。虽然葡聚糖 - P对佛波酯（PMA）诱导的超氧化物释放和IL - 1水平的影响在C3H/HeJ和C3H/HeN小鼠的巨噬细胞中相似，但葡聚糖 - F在增强C3H/HeJ小鼠中PMA诱导的超氧化物释放或增加IL - 1水平方面无效。因此，（1）葡聚糖 - F不能模拟葡聚糖 - P对调理红细胞和乳胶珠吞噬作用的影响；（2）虽然C3H/HeJ小鼠的巨噬细胞对葡聚糖 - P反应正常（与C3H/HeN巨噬细胞相比），但它们对葡聚糖 - F反应低下。这些发现表明，葡聚糖 - P激活巨噬细胞涉及与葡聚糖 - F激活的途径不同（或额外）的途径。
