Adachi Y, Ohno N, Yadomae T
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan.
Biol Pharm Bull. 1993 May;16(5):462-7. doi: 10.1248/bpb.16.462.
Effects of the pretreatment of murine peritoneal macrophages with several polysaccharides on the production of H2O2 induced with unopsonized zymosan were examined. Pretreatment with most of (1-->3)-beta-D-glucans for 6 h at 37 degrees C inhibited the zymosan-mediated H2O2 production by macrophages. The phorbol myristate acetate (PMA)-mediated H2O2 production was not affected by the pretreatment. The pretreatment of macrophages with (1-->3)-beta-D-glucans decreased the ability to ingest unopsonized zymosan, but did not affect the ingestion of IgG-coated sheep red blood cells (IgG-SRBC). These results suggested that the pretreatment with (1-->3)-beta-D-glucans interfered with the interaction of macrophages to zymosan and that the occupation of the receptor for the (1-->3)-beta-D-glucans inhibited zymosan-mediated production of H2O2 by macrophages. Chemical modification by substitution with carboxymethyl groups or hydroxyethyl groups of a (1-->6)-branched (1-->3)-beta-D-glucan reduced the inhibitory effect of pretreatment on zymosan-mediated H2O2 production. The above results indicated the possibility that murine peritoneal macrophages possess certain receptors for beta-anomeric glucans, and one ligand specificity of the receptors is to restrict the intact (1-->3)-beta-D-glucosyl back bone.
研究了几种多糖对小鼠腹腔巨噬细胞进行预处理后，对未调理酵母聚糖诱导产生过氧化氢的影响。大多数（1→3）-β-D-葡聚糖在37℃下预处理6小时可抑制巨噬细胞由酵母聚糖介导的过氧化氢产生。佛波酯（PMA）介导的过氧化氢产生不受预处理的影响。用（1→3）-β-D-葡聚糖预处理巨噬细胞会降低摄取未调理酵母聚糖的能力，但不影响摄取IgG包被的绵羊红细胞（IgG-SRBC）。这些结果表明，用（1→3）-β-D-葡聚糖预处理会干扰巨噬细胞与酵母聚糖的相互作用，并且（1→3）-β-D-葡聚糖受体的占据会抑制巨噬细胞由酵母聚糖介导的过氧化氢产生。用羧甲基或羟乙基取代对（1→6）分支的（1→3）-β-D-葡聚糖进行化学修饰可降低预处理对酵母聚糖介导的过氧化氢产生的抑制作用。上述结果表明，小鼠腹腔巨噬细胞可能具有某些β-异头葡聚糖受体，并且该受体的一种配体特异性是限制完整的（1→3）-β-D-葡糖基主链。
