Gururangan S, Dorman A, Ball R, Curran B, Leader M, Breatnach F, O'Meara A
Children's Research Centre, Our Lady's Hospital for Sick Children, Dublin, Ireland.
J Clin Pathol. 1992 Jun;45(6):498-501. doi: 10.1136/jcp.45.6.498.
To compare flow cytometry (FCM) with image analysis (IA) in the DNA quantitation of Wilms' tumour (WT) and to correlate data so obtained with recognised clinical and pathological prognostic parameters.
Thirty six patients with histologically proved WT diagnosed between 1980-89 were investigated. Fifteen patients had stage I disease, 10 stage II, six stage III, two stage IV and three stage V. Suspension of nuclei obtained by pepsin digestion of paraffin wax embedded tumour tissue was analysed using a FAC-Scan flow cytometer, and a CAS-100 image analyser.
Tumours were concordant in most instances, however, IA identified aneuploidy in two tumour samples which were diploid by FCM. Aneuploidy was detected in 5/33 tumours with favourable histology and 3/3 with unfavourable histology. Three of 28 patients with Stage I, II and V disease and 5/8 patients with stage III and IV had aneuploid tumours. All patients with unfavourable histology died of disease. In the group with favourable histology, 4/5 patients with aneuploid tumours developed recurrent disease compared with 1/27 diploid tumours (p less than 0.0001).
Ploidy may be a useful additional prognostic indicator in Wilms' tumour with favourable histology. Larger scale studies are needed to confirm the relation of ploidy to survival in early stage WT.
比较流式细胞术（FCM）与图像分析（IA）在肾母细胞瘤（WT）DNA定量分析中的应用，并将所得数据与公认的临床和病理预后参数进行关联。
对1980年至1989年间经组织学证实为WT的36例患者进行研究。15例为I期疾病，10例为II期，6例为III期，2例为IV期，3例为V期。使用FAC-Scan流式细胞仪和CAS-100图像分析仪对通过胃蛋白酶消化石蜡包埋肿瘤组织获得的细胞核悬液进行分析。
在大多数情况下，肿瘤情况是一致的，然而，IA在两个FCM检测为二倍体的肿瘤样本中识别出非整倍体。在组织学良好的33个肿瘤中有5个检测到非整倍体，在组织学不良的3个肿瘤中有3个检测到非整倍体。28例I、II和V期疾病患者中有3例以及III和IV期的8例患者中有5例患有非整倍体肿瘤。所有组织学不良的患者均死于该疾病。在组织学良好的组中，5例非整倍体肿瘤患者中有4例发生复发性疾病，而27例二倍体肿瘤患者中有1例发生复发性疾病（p小于0.0001）。
倍性可能是组织学良好的肾母细胞瘤中一个有用的额外预后指标。需要进行更大规模的研究来证实早期WT中倍性与生存的关系。
