Yanagihara T, Matsumoto I, Kawana R
Department of Bacteriology, School of Medicine, Iwate Medical University.
Kansenshogaku Zasshi. 1992 Apr;66(4):484-9. doi: 10.11150/kansenshogakuzasshi1970.66.484.
We carried out cloning of RD cells and obtained cloned cell line RD-9H8, which is highly sensitive to enteroviruses. Cytopathic effect (CPE) of RD-9H8 cells was more remarkable than that of original RD cells. The RD-9H8 cells were used to measure the neutralizing antibodies to coxsackievirus group B in sera from 93 medical students collected in Oct. 1990 at Iwate Medical University. The proportions of confirmed positive sera were coxsackievirus type B1 44.1%, type B2 50.5%, type B3 46.2%, type B4 64.5%, type B5 53.8% and type B6 4.4%.
我们对RD细胞进行了克隆，获得了对肠道病毒高度敏感的克隆细胞系RD - 9H8。RD - 9H8细胞的细胞病变效应（CPE）比原始RD细胞更显著。利用RD - 9H8细胞检测了1990年10月在岩手医科大学收集的93名医学生血清中针对B组柯萨奇病毒的中和抗体。确诊阳性血清的比例分别为：B1型柯萨奇病毒44.1%、B2型50.5%、B3型46.2%、B4型64.5%、B5型53.8%和B6型4.4%。
