Tabachnick W J
Arthropod-borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, Wyoming 82071.
J Med Entomol. 1992 May;29(3):384-94. doi: 10.1093/jmedent/29.3.384.
Seven Colorado populations of the bluetongue virus vector Culicoides varipennis (Coquillett) were analyzed for genetic variation at 19-21 isozyme loci. Permanent populations, which overwinter as larvae, showed little temporal genetic change at 19 loci. PGD and MDH showed seasonal changes in gene frequencies, attributable to selection at two permanent populations. Two temporary populations showed low heterozygosity compared with permanent populations. Independent estimates of gene flow, calculated using FST and the private allele method, were Nm* = 2.15 and 6.95, respectively. Colorado C. variipennis permanent populations showed high levels of gene flow which prevented significant genetic differentiation due to genetic drift. Temporary populations showed significant gene frequency differences from nearby permanent populations due to the "founder effect" associated with chance colonization.
对科罗拉多州的七个蓝舌病病毒传播媒介——库蠓（Culicoides varipennis，Coquillett）种群，在19 - 21个同工酶位点上的遗传变异进行了分析。以幼虫形式越冬的永久性种群，在19个位点上几乎没有随时间发生遗传变化。磷酸葡萄糖脱氢酶（PGD）和苹果酸脱氢酶（MDH）的基因频率呈现季节性变化，这归因于两个永久性种群中的选择作用。与永久性种群相比，两个临时性种群显示出较低的杂合性。使用FST和私有等位基因方法计算得出的基因流独立估计值，分别为Nm* = 2.15和6.95。科罗拉多州的库蠓永久性种群显示出高水平的基因流，这阻止了由于遗传漂变导致的显著遗传分化。由于与偶然定殖相关的“奠基者效应”，临时性种群与附近的永久性种群表现出显著的基因频率差异。
