Holbrook F R, Tabachnick W J
Arthropod-Borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, WY 82071-3965, USA.
J Med Entomol. 1995 Jul;32(4):413-9. doi: 10.1093/jmedent/32.4.413.
Genetic relationships were examined among 24 collections, representing 23 populations of Culicoides variipennis (Coquillett) using isozyme electrophoresis of 11 protein encoding loci. The populations were collected from alkaline or fresh water larval habitats in California. Distance analysis demonstrated that C. v. occidentalis Wirth and Jones and C. v. sonorensis Wirth and Jones are genetically distinct. All C. v. occidentalis were geographically isolated from each other in highly alkaline or saline larval habitats, whereas C. v. sonorensis populations were collected from artificial freshwater habitats that were polluted with organic wastes. Higher levels of gene flow were found between C. v. sonorensis populations than from C. v. sonorensis populations to nearby C. v. occidentalis populations, indicative of genetic isolation between subspecies. Northern California C. v. sonorensis were genetically distinguishable from southern California C. v. sonorensis. The relationship between this variation and bluetongue disease epidemiology in California is discussed.
利用11个蛋白质编码位点的同工酶电泳技术，对代表23个变异库蠓（Coquillett）种群的24个样本进行了遗传关系研究。这些种群采自加利福尼亚州的碱性或淡水幼虫栖息地。距离分析表明，西方库蠓（C. v. occidentalis）Wirth和Jones以及索诺拉库蠓（C. v. sonorensis）Wirth和Jones在遗传上是不同的。所有西方库蠓在高碱性或含盐的幼虫栖息地中彼此地理隔离，而索诺拉库蠓种群采自被有机废物污染的人工淡水栖息地。索诺拉库蠓种群之间的基因流水平高于从索诺拉库蠓种群到附近西方库蠓种群的基因流水平，这表明亚种之间存在遗传隔离。北加利福尼亚的索诺拉库蠓在遗传上与南加利福尼亚的索诺拉库蠓不同。本文还讨论了这种变异与加利福尼亚蓝舌病流行病学之间的关系。
