Nolan L K, Wooley R E, Cooper R K
Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Avian Dis. 1992 Apr-Jun;36(2):398-402.
The role of complement resistance in the virulence of an avian Escherichia coli isolate was examined with transposon mutagenesis. A suicide plasmid containing a kanamycin-encoding mini-transposon was used to transform a virulent complement-resistant avian E. coli isolate. A less resistant mutant was identified that contained a transposon insertion in a plasmid and in the chromosome. This loss of complement resistance was associated with a drop in virulence in an embryo assay. No other phenotypic changes were detected in the mutant. These results suggest that complement resistance is associated with the virulence of this organism.
利用转座子诱变技术研究了补体抗性在禽源大肠杆菌分离株毒力中的作用。使用含有编码卡那霉素的微型转座子的自杀质粒来转化具有补体抗性的禽源大肠杆菌强毒株。鉴定出一株抗性较低的突变体，其在质粒和染色体中均有转座子插入。这种补体抗性的丧失与胚胎试验中毒力的下降有关。在该突变体中未检测到其他表型变化。这些结果表明补体抗性与该生物体的毒力相关。
