Nolan L K, Wooley R E, Giddings C W, Brown J
Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo 58105.
Avian Dis. 1994 Jan-Mar;38(1):146-50.
A virulent, complement-resistant avian Escherichia coli isolate and its avirulent, complement-sensitive, transposon-insertion mutant were compared for the purpose of revealing structures associated with complement resistance. Both had a smooth lipopolysaccharide layer, contained traT, and lacked a capsule, but the mutant possessed a 16.2-kilodalton outer-membrane protein (OMP) not present in the wild-type. This protein may be the product of a coding region interrupted by transposon insertion. Such results suggest that an OMP greater than 16.2 kilodaltons in size may be responsible for the complement resistance and virulence of this wild-type E. coli.
为了揭示与补体抗性相关的结构，对一株强毒、抗补体的禽源大肠杆菌分离株及其无毒、补体敏感的转座子插入突变体进行了比较。两者都有光滑的脂多糖层，含有traT，且没有荚膜，但突变体拥有一种野生型中不存在的16.2千道尔顿外膜蛋白（OMP）。这种蛋白质可能是被转座子插入打断的编码区的产物。这些结果表明，大小大于16.2千道尔顿的OMP可能是这种野生型大肠杆菌补体抗性和毒力的原因。
