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大鼠肝脏离体线粒体对嘌呤和嘧啶核苷的磷酸化作用。

Phosphorylation of purine and pyrimidine nucleosides by isolated rat liver mitochondria.

作者信息

Inaba K, Oda T

出版信息

Acta Med Okayama. 1975 Oct;29(5):367-75.

PMID:132087
Abstract

Formation of 5'-AMP, 5'-GMP, 5'-CMP and 5'UMP was confirmed in isolated rat liver mitochondria incubated with alpha-ketoglutarate, inorganic phosphate, purine nucleoside and pyrimidine nucleoside. Increased incorporation of 32Pi into ATP, GTP and UTP was observed by adding purine- and pyrimidine nucleosides. The phosphorylation of nucleosides was inhibited severely by arsenite and affected slightly by the addition of nuclear or post-mitochondrial fraction.

摘要

在与α-酮戊二酸、无机磷酸盐、嘌呤核苷和嘧啶核苷一起孵育的离体大鼠肝线粒体中,证实了5'-AMP、5'-GMP、5'-CMP和5'-UMP的形成。通过添加嘌呤和嘧啶核苷,观察到32Pi掺入ATP、GTP和UTP的量增加。亚砷酸盐严重抑制核苷的磷酸化,而添加细胞核或线粒体后组分对其影响较小。

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