Inaba K, Oda T
Acta Med Okayama. 1977 Apr;31(2):141-6.
Mitochondrial RNA (mtRNA) was synthesized from purine and pyrimidine nucleosides in coupling with oxidative phosphorylation using isolated mitochondria. The in vivo synthesized mtRNA was adenine-uracil rich and sedimented at about 20 S by sucrose density gradient centrifugation. A major part of the newly synthesized mtRNA was shown to be poly (A)-containing RNA by the resistance to the digestion with pancreatic RNase and RNase T1 and the affinity to poly (U)-Sepharose columns or Millipore filters.
利用分离的线粒体，通过与氧化磷酸化偶联，从嘌呤和嘧啶核苷合成线粒体RNA（mtRNA）。体内合成的mtRNA富含腺嘌呤-尿嘧啶，通过蔗糖密度梯度离心在约20S处沉降。新合成的mtRNA的主要部分通过对胰核糖核酸酶和核糖核酸酶T1消化的抗性以及对聚（U）-琼脂糖柱或微孔滤膜的亲和力显示为含聚（A）的RNA。
