Verbeeck M A, Mummery C L, Feijen A, Burbach J P
Rudolf Magnus Institute, Medical Faculty, University of Utrecht, The Netherlands.
Pathobiology. 1992;60(3):127-35. doi: 10.1159/000163711.
The presence of 3 different neuropeptide mRNAs with a strict cell-specific expression in vivo was investigated in 13 tumor cell lines from neuroendocrine and in 23 tumor cell lines from non-neuroendocrine origin. Northern blots showed no expression of mRNA for vasopressin (VP) in the 36 tested cell lines. Very low oxytocin (OT) mRNA hybridization signals were detected in the rat pituitary tumor cell line GH4C2 and the rat pancreas tumor cell line RIN5. Both the rat pituitary tumor cell line AtT-20 and the human myeloid leukemia cell line K562, contained proopiomelanocortin (POMC) mRNA. The low incidence of VP, OT and POMC gene expression in the tested tumor cell lines was not influenced by treatments inducing differentiation. In contrast, the cholecystokinin (CCK) gene which is widely present in nervous and endocrine systems was abundantly expressed in the human primitive neuroepithelioma cell line SK-N-MC and its clonal derivative SK-N-MC-IX-C. The results indicate that the expression of neuropeptide genes is very rare in tumor cell lines. The lack of expression in undifferentiated cells agrees with the appearance of expression after day 13 of the embryogenesis when maturation of neurons begins.
在13种神经内分泌来源的肿瘤细胞系和23种非神经内分泌来源的肿瘤细胞系中，研究了3种不同神经肽mRNA在体内严格的细胞特异性表达情况。Northern印迹显示，在36种受试细胞系中均未检测到血管加压素（VP）mRNA的表达。在大鼠垂体肿瘤细胞系GH4C2和大鼠胰腺肿瘤细胞系RIN5中，检测到极低的催产素（OT）mRNA杂交信号。大鼠垂体肿瘤细胞系AtT-20和人髓样白血病细胞系K562均含有阿黑皮素原（POMC）mRNA。受试肿瘤细胞系中VP、OT和POMC基因表达的低发生率不受诱导分化处理的影响。相反，在神经系统和内分泌系统中广泛存在的胆囊收缩素（CCK）基因，在人原始神经上皮瘤细胞系SK-N-MC及其克隆衍生物SK-N-MC-IX-C中大量表达。结果表明，神经肽基因在肿瘤细胞系中的表达非常罕见。未分化细胞中缺乏表达与胚胎发育第13天神经元开始成熟后表达的出现情况相符。
