Verbeeck M A, Burbach J P
Rudolf Magnus Institute, Medical Faculty, University of Utrecht, The Netherlands.
FEBS Lett. 1990 Jul 30;268(1):88-90. doi: 10.1016/0014-5793(90)80979-s.
The cholinergic human neuroepithelioma cell line SK-N-MCIXC expressed mRNAs for the neuropeptides cholecystokinin (CCK), neuropeptide Y, gastrin-releasing peptide (GRP) and enkephalin. The CCK transcript of about 800 nt was present at very high levels and CCK-like peptides immunoreactive to a C-terminal CCK octapeptide antiserum were present in the cell line and its medium. This clonal neuronal cell line provides a unique model system to identify cis- and trans-acting factors responsible for neuron-specific expression and regulation of the CCK gene. Furthermore, the pluripotent properties of the undifferentiated cell line may open studies on neuronal differentiation at the level of co-expression of neuropeptides and transmitters.
胆碱能人类神经上皮瘤细胞系SK-N-MCIXC表达了神经肽胆囊收缩素（CCK）、神经肽Y、胃泌素释放肽（GRP）和脑啡肽的信使核糖核酸。约800个核苷酸的CCK转录本以非常高的水平存在，并且该细胞系及其培养基中存在与C末端CCK八肽抗血清发生免疫反应的CCK样肽。这个克隆神经元细胞系提供了一个独特的模型系统，以鉴定负责CCK基因神经元特异性表达和调控的顺式和反式作用因子。此外，未分化细胞系的多能特性可能开启关于神经肽和递质共表达水平上神经元分化的研究。
