Potier M C, Spillantini M G, Carter N P
Medical Research Council, Laboratory of Molecular Biology, Cambridge, England.
DNA Seq. 1992;2(4):211-8. doi: 10.3109/10425179209020805.
The rat glutamate receptor is a 907 amino acid transmembrane protein. Using the rat GluR1 cDNA as a probe, we have isolated cDNA clones from a human hippocampal cDNA library. Sequence of a full length cDNA clone revealed 98.2% and 89.4% identity to the rat sequence at the amino acid and nucleotide levels respectively. The human cDNA clone detected an RNA transcript in human cerebral cortex, hippocampus and cerebellum, similar to that seen in rat. In situ hybridization experiments showed that human GluR1 mRNA is present in granule and pyramidal cells in the hippocampal formation and that there is no apparent difference of distribution between control patient and patient with Alzheimer's disease. Dot blot analysis of flow-sorted human chromosomes showed that the GluR1 gene maps to chromosome 5.
大鼠谷氨酸受体是一种由907个氨基酸组成的跨膜蛋白。我们以大鼠GluR1 cDNA为探针，从人海马cDNA文库中分离出了cDNA克隆。一个全长cDNA克隆的序列显示，其在氨基酸水平和核苷酸水平上与大鼠序列的同源性分别为98.2%和89.4%。该人cDNA克隆在人大脑皮层、海马和小脑中检测到一种RNA转录本，与在大鼠中观察到的相似。原位杂交实验表明，人GluR1 mRNA存在于海马结构的颗粒细胞和锥体细胞中，且对照患者与阿尔茨海默病患者之间的分布没有明显差异。对流式分选的人类染色体进行斑点印迹分析表明，GluR1基因定位于5号染色体。
