Hutton M L, Harvey R J, Barnard E A, Darlison M G
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, UK.
FEBS Lett. 1991 Nov 4;292(1-2):111-4. doi: 10.1016/0014-5793(91)80846-u.
A full-length cDNA which encodes a putative glutamate receptor polypeptide was isolated from the pond snail Lymnaea stagnalis, using a short stretch of exonic sequence and two variants of the polymerase chain reaction. In this first comparison of invertebrate and vertebrate glutamate receptor sequences, the mature molluscan polypeptide, which comprises 898 amino acids and has a predicted Mr of 100,913, displays between 37% and 46% amino-acid identity to the rat ionotropic glutamate receptor subunits, GluR1 to GluR6.
利用一段外显子序列和聚合酶链反应的两种变体，从椎实螺中分离出一个编码假定谷氨酸受体多肽的全长互补DNA。在对无脊椎动物和脊椎动物谷氨酸受体序列的首次比较中，成熟的软体动物多肽由898个氨基酸组成，预测分子量为100,913，与大鼠离子型谷氨酸受体亚基GluR1至GluR6的氨基酸同一性在37%至46%之间。
