Grevelding C, Becker D, Kunze R, von Menges A, Fantes V, Schell J, Masterson R
Max-Planck-Institut für Züchtungsforschung, Cologne, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6085-9. doi: 10.1073/pnas.89.13.6085.
Overexpression of the Activator (Ac) transposase gene in Arabidopsis thaliana resulted in a minimal germinal transposition frequency of 27% in which independent Dissociation (Ds) transposition events were observed. Molecular analysis of 45 F1 generation Ac/Ds plants indicated that high rates of somatic excision had occurred, and independent germinal insertions were identified in F2 generation progeny plants. A tandem cauliflower mosaic virus (CaMV) promoter fused to two different Ac coding sequences significantly increased the rate of Ds transposition. The CaMV-Ac fusions activated single and multiple copies of two different Ds elements, DsDHFR and Ds35S-1, and reciprocal crosses resulted in similar transposition frequencies. The improved rate of independent germinal transposition observed makes Arabidopsis an ideal system for insertional mutagenesis.
激活子（Ac）转座酶基因在拟南芥中的过表达导致了最低27%的生殖转座频率，在此过程中观察到了独立的解离（Ds）转座事件。对45株F1代Ac/Ds植株的分子分析表明，体细胞切除率很高，并且在F2代子代植株中鉴定出了独立的生殖插入。与两个不同Ac编码序列融合的串联花椰菜花叶病毒（CaMV）启动子显著提高了Ds转座率。CaMV-Ac融合激活了两个不同Ds元件DsDHFR和Ds35S-1的单拷贝和多拷贝，正反交产生了相似的转座频率。观察到的独立生殖转座率的提高使拟南芥成为插入诱变的理想系统。
Zotero 插件