无钙溶液中参与大鼠子宫收缩的介质。

The effect of (Na+ + K+)-ATPase inhibitor ouabain (10(-5)-3 x 10(-4) M), and the (Ca2+ + Mg2+)-ATPase inhibitors vanadate (6 x 10(-6)-6 x 10(-4) M), oxytocin (2 x 10(-9)-4 x 10(-8) M, and prostaglandin F2 alpha (PGF2 alpha, 10(-7)-6 x 10(-6) M) were assayed on rat uterus incubated in Ca-free medium. 2. Vanadate, oxytocin and PGF2 alpha, but not ouabain, induced contractions in a dose-dependent way (ED50: 7.5 +/- 0.03 x 10(-5) M; 6.5 +/- 0.064 x 10(-9) M and 3.8 +/- 0.085 x 10(-7) M). 3. Vanadate (3 x 10(-4) M) and oxytocin (OT, 10 mU/ml = 2 x 10(-8) M)-induced tonic contraction were not modified by nifedipine (10(-10)-10(-6) M), monensin (10(-5)-3 x 10(-4) M) or amiloride (10(-5)-10(-3) M). 4. The intracellular calcium release inhibitors TMB-8 (10(-6)-10(-4) M) and dantrolene (3 x 10(-6)-10(-4) M), and the prostaglandin release inhibitor indomethacin (3 x 10(-8)-6 x 10(-5) M) relaxed the vanadate and OT-induced tonic contractions. 5. The calmodulin inhibitors trifluoperazine (3 x 10(-5)-3 x 10(-4) M), bepridil (10(-8)-3 x 10(-4) M), calmidazolium (10(-7)-10(-4) M) and W-7 (10(-7)-10(-5) M) also relaxed the vanadate and OT-induced tonic contractions. 6. Our results suggest that oxytocin and vanadate-induced contractions on rat uterus in Ca-free medium could be produced by release of prostaglandins and intracellular calcium, and mediated by calmodulin.

在无钙培养基中孵育的大鼠子宫上，检测了（Na⁺ + K⁺）-ATP酶抑制剂哇巴因（10⁻⁵ - 3×10⁻⁴ M）、（Ca²⁺ + Mg²⁺）-ATP酶抑制剂钒酸盐（6×10⁻⁶ - 6×10⁻⁴ M）、催产素（2×10⁻⁹ - 4×10⁻⁸ M）和前列腺素F2α（PGF2α，10⁻⁷ - 6×10⁻⁶ M）的作用。2. 钒酸盐、催产素和PGF2α可诱导剂量依赖性收缩（半数有效剂量：7.5±0.03×10⁻⁵ M；6.5±0.064×10⁻⁹ M和3.8±0.085×10⁻⁷ M），但哇巴因无此作用。3. 硝苯地平（10⁻¹⁰ - 10⁻⁶ M）、莫能菌素（10⁻⁵ - 3×10⁻⁴ M）或阿米洛利（10⁻⁵ - 10⁻³ M）不改变钒酸盐（3×10⁻⁴ M）和催产素（OT，10 mU/ml = 2×10⁻⁸ M）诱导的强直性收缩。4. 细胞内钙释放抑制剂TMB-8（10⁻⁶ - 10⁻⁴ M）和丹曲林（3×10⁻⁶ - 10⁻⁴ M）以及前列腺素释放抑制剂吲哚美辛（3×10⁻⁸ - 6×10⁻⁵ M）可缓解钒酸盐和OT诱导的强直性收缩。5. 钙调蛋白抑制剂三氟拉嗪（3×10⁻⁵ - 3×10⁻⁴ M）、苄普地尔（10⁻⁸ - 3×10⁻⁴ M）、卡米达唑（10⁻⁷ - 10⁻⁴ M）和W-7（10⁻⁷ - 10⁻⁵ M）也可缓解钒酸盐和OT诱导的强直性收缩。6. 我们的结果表明，在无钙培养基中，催产素和钒酸盐诱导的大鼠子宫收缩可能是由前列腺素和细胞内钙的释放引起的，并由钙调蛋白介导。

Mediators involved in the rat uterus contraction in calcium-free solution.