钒酸盐对大鼠睾丸白膜的影响。

Effects of vanadate in testicular capsule of the rat.

Sanchez M, Andrés-Trelles F, Hidalgo A

Laboratorio de Farmacologia (Departamento de Medicina), Facultad de Medicina, Universidad de Oviedo, Spain.

Gen Pharmacol. 1991;22(3):499-503. doi: 10.1016/0306-3623(91)90013-v.

Abstract

The effects of sodium orthovanadate (vanadate. 10(-5) to 3 x 10(-4) M) on testicular capsule of the rat, and the modifications of these effects by the calcium chelator EGTA (2mM), the calcium entry blockers verapamil (5 X 10(-5) M), nifedipine (10(-5 M) and diltiazem (5 x 10(-5) M), the (Na+ + K+)-ATPase inhibitor ouabain, the Na+/Ca2+ exchange inhibitor amiloride, and the calmodulin antagonists trifluoperazine (10(-4) M) and W-7 (5 x 10(-5) M) have been studied. 2. Vanadate induced contraction of the rat testicular capsule in a dose-dependent way (ED50: 82.8 +/- 7.4 x 10(-6) M). 3. The contraction induced by vanadate (3 and 30 x 10(-5) M) were abolished by EGTA and not modified by verapamil, nifedipine, flunarizine or diltiazem. 4. Amiloride (1 and 5 x 10(-5) M), but not ouabain (5 x 10(-5) and 10(-4) M), inhibit in a dose-dependent way the contraction induced by two doses of vanadate (3 and 30 x 10(-5) M). 5. Trifluoperazine and W-7 significantly inhibit the contraction of testicular capsule to 3 and 30 x 10(-5) M vanadate.

摘要

研究了原钒酸钠（钒酸盐，浓度为10⁻⁵至3×10⁻⁴M）对大鼠睾丸白膜的作用，以及钙螯合剂乙二醇双四乙酸（EGTA，2mM）、钙通道阻滞剂维拉帕米（5×10⁻⁵M）、硝苯地平（10⁻⁵M）和地尔硫䓬（5×10⁻⁵M）、（钠⁺ + 钾⁺）-ATP酶抑制剂哇巴因、钠/钙交换抑制剂阿米洛利以及钙调蛋白拮抗剂三氟拉嗪（10⁻⁴M）和W-7（5×10⁻⁵M）对这些作用的影响。2. 钒酸盐以剂量依赖性方式诱导大鼠睾丸白膜收缩（半数有效剂量：82.8±7.4×10⁻⁶M）。3. EGTA可消除钒酸盐（3和30×10⁻⁵M）诱导的收缩，而维拉帕米、硝苯地平、氟桂利嗪或地尔硫䓬对此无影响。4. 阿米洛利（1和5×10⁻⁵M）而非哇巴因（5×10⁻⁵和10⁻⁴M）以剂量依赖性方式抑制两剂量钒酸盐（3和30×10⁻⁵M）诱导的收缩。5. 三氟拉嗪和W-7可显著抑制睾丸白膜对3和30×10⁻⁵M钒酸盐的收缩反应。