Maurer J, Mann W, Baggelmann M
ENT Department, University of Mainz, Federal Republic of Germany.
Eur Arch Otorhinolaryngol. 1992;249(3):176-80. doi: 10.1007/BF00183496.
The activity of Ca(2+)-ATPase in the inner ear of the guinea pig was studied ultracytochemically by the lead citrate reaction. The electron-dense reaction products as an expression of Ca(2+)-ATPase activity were localized in endolymphatic cells of Reissner's membrane, in outer and inner hair cells and in some supporting cells. The main finding was the difference in the localization of Ca(2+)-ATPase in outer and inner hair cells. In the latter cells the activity sites were mainly intracellular and in apical membrane specializations, whereas in the outer hair cells the enzyme was localized in the apical membrane specializations and the basolateral plasma membrane.
通过柠檬酸铅反应，采用超微细胞化学方法研究了豚鼠内耳中Ca(2+)-ATP酶的活性。作为Ca(2+)-ATP酶活性表现的电子致密反应产物定位于Reissner膜的内淋巴细胞、外毛细胞和内毛细胞以及一些支持细胞中。主要发现是Ca(2+)-ATP酶在外毛细胞和内毛细胞中的定位存在差异。在内毛细胞中，活性位点主要位于细胞内和顶端膜特化结构中，而在外毛细胞中，该酶定位于顶端膜特化结构和基底外侧质膜中。
