通过80 kDa二酰基甘油激酶的表达消除缓激肽诱导的ras转化成纤维细胞中的钙振荡。
Abolishment of bradykinin-induced calcium oscillations in ras-transformed fibroblasts by the expression of 80 kDa diacylglycerol kinase.
作者信息
Fu T, Sugimoto Y, Okano Y, Kanoh H, Nozawa Y
机构信息
Department of Biochemistry, Gifu University School of Medicine, Japan.
出版信息
FEBS Lett. 1992 Aug 3;307(3):301-4. doi: 10.1016/0014-5793(92)80700-q.
Our previous study showed bradykinin-induced periodic Ca2+ changes (Ca2+ oscillations) in v-Ki-ras-transformed NIH/3T3 (DT) cells in which protein kinase C (PKC) activity is partially down-regulated by a sustained high level of 1,2-diacylglycerol (DAG) [FEBS Lett. (1991) 281, 263-266]. In the present study, DAG kinase with 80 kDa mass (80K DGK) has been successfully transfected in DT cells, which exhibited enhanced cellular DAG kinase activities, decreased cellular DAG contents, and increased PKC activities compared to the control vector-transfected cells. Furthermore, these DGK-transfectants showed strong inhibition in bradykinin-induced Ca2+ oscillations. The results suggest that the sustained DAG increase down-regulates the PKC activity, thereby leading to the induction of Ca2+ oscillations in DT cells.
我们之前的研究表明，缓激肽可诱导v-Ki-ras转化的NIH/3T3（DT）细胞发生周期性Ca2+变化（Ca2+振荡），在这些细胞中，蛋白激酶C（PKC）活性因持续高水平的1,2 -二酰甘油（DAG）而部分下调[《欧洲生物化学学会联合会快报》（1991年）281, 263 - 266]。在本研究中，80 kDa的二酰甘油激酶（80K DGK）已成功转染至DT细胞中，与转染对照载体的细胞相比，这些细胞表现出增强的细胞二酰甘油激酶活性、降低的细胞DAG含量以及增加的PKC活性。此外，这些DGK转染细胞在缓激肽诱导的Ca2+振荡中表现出强烈抑制作用。结果表明，持续的DAG增加会下调PKC活性，从而导致DT细胞中Ca2+振荡的诱导。
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