Ahmad I, Steggles A W, Finkelstein J A
Department of Anatomy, Northeastern Ohio Universities College of Medicine, Rootstown 44272.
Int J Obes Relat Metab Disord. 1992 Jun;16(6):435-41.
In order to investigate whether the impaired GH secretion associated with obesity is due to a pituitary disorder we studied GH mRNA levels by in situ hybridization in genetically obese and lean Zucker rats. The levels of GH mRNA were at least two fold lower in obese rats in comparison to that in lean controls as quantified by both the scanning of autoradiographs of tissue sections and Northern blot analysis. Quantification of somatotrophs revealed no significant difference in their number between lean and obese rat pituitaries. It is therefore likely that the attenuated GH mRNA levels in genetically obese Zucker rats are due to a decrease in GH transcripts per somatotroph rather than a result of a pituitary defect involving a preferential decrease in somatotroph population.
为了研究与肥胖相关的生长激素（GH）分泌受损是否归因于垂体疾病，我们通过原位杂交技术研究了遗传性肥胖和瘦型 Zucker 大鼠的 GH mRNA 水平。通过对组织切片放射自显影片的扫描和 Northern 印迹分析定量显示，肥胖大鼠的 GH mRNA 水平与瘦型对照相比至少低两倍。对生长激素细胞的定量分析表明，瘦型和肥胖大鼠垂体中生长激素细胞的数量没有显著差异。因此，遗传性肥胖 Zucker 大鼠中 GH mRNA 水平的降低可能是由于每个生长激素细胞中 GH 转录本的减少，而不是由于涉及生长激素细胞数量优先减少的垂体缺陷所致。
