Mack K J, Cortner J, Mack P, Farnham P J
Department of Neurology, University of Wisconsin, Madison 53705.
Brain Res Mol Brain Res. 1992 Jun;14(1-2):117-23. doi: 10.1016/0169-328x(92)90018-7.
krox 20 is an inducible immediate early response gene. To determine if krox 20 has a physiological role in the adult central nervous system (CNS), this study sought to demonstrate the presence of krox 20 in adult rat brain. RNA analysis showed the presence of krox 20 transcripts in the CNS, including the cortex. Polyclonal antibodies to a Krox 20 fusion protein demonstrated 79 and 55 kDa antigens in nuclear CNS homogenates. Neither RNA nor protein analysis was able to demonstrate an induction of krox 20 by a seizure at times when other immediate early response genes are known to be induced. Immunohistochemical analysis revealed staining at several levels throughout the nervous system. This staining was predominantly nuclear, consistent with the role of krox 20 as a transcription factor. These data show that krox 20 is present in the adult CNS, yet differs in response to stimuli as compared to other related transcription factors with a zinc finger motif, such as NGFI-A and NGFI-C.
Krox 20是一种可诱导的即刻早期反应基因。为了确定Krox 20在成体中枢神经系统（CNS）中是否具有生理作用，本研究试图证明其在成年大鼠脑中的存在。RNA分析表明中枢神经系统（包括皮层）中存在Krox 20转录本。针对Krox 20融合蛋白的多克隆抗体在中枢神经系统核匀浆中显示出79 kDa和55 kDa的抗原。在已知其他即刻早期反应基因会被诱导的时间点，RNA和蛋白质分析均未能证明癫痫发作可诱导Krox 20。免疫组织化学分析显示在整个神经系统的多个层面均有染色。这种染色主要位于细胞核，这与Krox 20作为转录因子的作用一致。这些数据表明Krox 20存在于成体中枢神经系统中，但与其他具有锌指基序的相关转录因子（如NGFI - A和NGFI - C）相比，其对刺激的反应有所不同。
