超氧阴离子（O2-）可能在铁诱导的微粒体脂质过氧化过程中在内膜大量生成。

A high involvement of O2- possibly generated in inner membranes for iron-induced microsomal lipid peroxidation.

作者信息

Koga S, Nakano M

机构信息

Photon Medical Research Center, Hamamatsu University School of Medicine, Shizuoka, Japan.

出版信息

Biochem Biophys Res Commun. 1992 Jul 31;186(2):1087-93. doi: 10.1016/0006-291x(92)90858-i.

DOI:10.1016/0006-291x(92)90858-i

PMID:1323282

Abstract

The lipid peroxidation of and the O2- generation by rat liver microsomes in the presence of NADPH or both NADPH and Fe3+ were determined by thiobarbituric acid-reacting substance formation and by chemiluminescence intensities with a cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3, 7-dihydroimidazo[1,2-a]pyrazin-3-one(MCLA), as a chemiluminescence probe. Judging from the experiments with various inhibitors on the O2- generation and the lipid peroxidation, O2- generated, at intramembranous site, by cytochrome P-450 system is considered to be highly involved in the iron-induced lipid peroxidation.

摘要

通过硫代巴比妥酸反应物质的形成以及使用海萤荧光素类似物2-甲基-6-（对甲氧基苯基）-3,7-二氢咪唑并[1,2-a]吡嗪-3-酮（MCLA）作为化学发光探针的化学发光强度，测定了在存在NADPH或同时存在NADPH和Fe³⁺的情况下大鼠肝微粒体的脂质过氧化和O₂⁻生成。从使用各种抑制剂对O₂⁻生成和脂质过氧化的实验判断，细胞色素P-450系统在膜内位点产生的O₂⁻被认为与铁诱导的脂质过氧化高度相关。