Tampo Y, Tsukamoto M, Yonaha M
Division of Environmental Hygiene, Hokkaido College of Pharmacy, Japan.
FEBS Lett. 1998 Jul 3;430(3):348-52. doi: 10.1016/s0014-5793(98)00689-9.
The antioxidant effect of 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-alpha]pyraz in-3-one (MCLA), a Cypridina luciferin analog that acts as a chemiluminescence probe to detect O2.-, was investigated. MCLA produced a lag in oxygen consumption induced by cumene hydroperoxide in microsomes or by 2,2'-azobis (2-amidinopropane) dihydrochloride in liposomes and disappeared during the duration of the lag. MCLA profoundly inhibited the propagation reaction in Fe2+-dependent lipid peroxidation in liposomes, and MCLA disappearance accompanied by suppression of oxygen consumption markedly occurred in liposomes susceptible to peroxidation. Thiobarbituric acid-reactive substances in all systems used were also suppressed by MCLA dose dependently. These results indicate that MCLA has an antioxidant property through scavenging free radicals.
研究了2-甲基-6-（对甲氧基苯基）-3,7-二氢咪唑并[1,2-α]吡嗪-3-酮（MCLA）的抗氧化作用，MCLA是一种海萤荧光素类似物，作为化学发光探针用于检测超氧阴离子（O2.-）。MCLA使微粒体中氢过氧化异丙苯或脂质体中2,2'-偶氮二（2-脒基丙烷）二盐酸盐诱导的耗氧出现滞后，且在滞后期间消失。MCLA显著抑制脂质体中Fe2+依赖性脂质过氧化的传播反应，在易发生过氧化的脂质体中明显出现MCLA消失并伴有耗氧抑制的情况。MCLA还剂量依赖性地抑制了所有所用体系中的硫代巴比妥酸反应性物质。这些结果表明，MCLA通过清除自由基具有抗氧化特性。
