[大鼠离体十二指肠黏膜渗透压刺激下钠钾ATP酶内源性调节因子的分泌]

[Secretion of endogenous regulators of Na K ATPase by isolated rat duodenum in osmotic stimulation of its mucous membrane].

作者信息

Iaremenko M S, Prokopenko O M, Vavilova H L, Kharlamova O M

出版信息

Fiziol Zh (1978). 1992 May-Jun;38(3):67-70.

Abstract

The experiments in vitro have shown that perfusion of the isolated duodenum cavity in rats by hypotonic (20 mosmol/l H2O) or hypertonic (500 mosmol/l H2O) solutions of NaCl and mannitol during 10 min stimulates secretion of endogenic factors (activators or inhibitors of enterocytes of Na+, K(+)-ATPase) into the serous incubation solution. Contact of the duodenum mucose surface with isotonic (300 mosmol/l H2O) solutions does not stimulate the secretion of this enzyme regulators. Endogenic substance are thermostable: they retain activity relative to Na+, K+ ATPase of enterocytes after heating (100 degrees C, 10 min duration) of serous incubation solutions.

摘要

体外实验表明，在10分钟内用低渗（20毫摩尔/升H₂O）或高渗（500毫摩尔/升H₂O）的氯化钠和甘露醇溶液灌注大鼠离体十二指肠腔，会刺激内源性因子（Na⁺、K⁺-ATP酶肠上皮细胞的激活剂或抑制剂）分泌到浆液孵育液中。十二指肠黏膜表面与等渗（300毫摩尔/升H₂O）溶液接触不会刺激这种酶调节剂的分泌。内源性物质具有热稳定性：在浆液孵育液加热（100℃，持续10分钟）后，它们对肠上皮细胞的Na⁺、K⁺-ATP酶仍保持活性。