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通过聚丙烯酰胺凝胶电泳鉴定的非典型轮状病毒基因组模式。

Atypical rotavirus genomic patterns identified by polyacrylamide gel electrophoresis.

作者信息

Lipson S M, Kaplan M H

机构信息

Jane and Dayton Brown and Dayton T. Brown, Jr, Virology Laboratory, Department of Medicine, North Shore University Hospital--Cornell University Medical College, Manhasset, New York.

出版信息

J Diarrhoeal Dis Res. 1992 Jun;10(2):97-100.

PMID:1323592
Abstract

Two atypical "short" rotavirus genomic profiles were detected in stool specimens following the testing of 84 children with diarrhoea. Both rotaviruses were identified as Group A, and confirmation was performed by blocking assay using antiserum against simian rotavirus SA-11. This report underscores the importance of screening on the molecular level to detect atypical or unusual rotaviruses that would normally proceed unrecognised by testing with any of the commercial antigen detection kits.

摘要

在对84名腹泻儿童的粪便标本进行检测后,发现了两种非典型的“短型”轮状病毒基因组图谱。两种轮状病毒均被鉴定为A组,并通过使用抗猴轮状病毒SA - 11的抗血清进行阻断试验来确认。本报告强调了在分子水平上进行筛查的重要性,以检测那些通常使用任何商业抗原检测试剂盒进行检测时无法识别的非典型或不寻常的轮状病毒。

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[Molecular epidemiology of rotavirus infections at units of children's hospitals].
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