[图库曼地区4年肠胃炎研究期间轮状病毒RNA的电泳类型]

[Electrophoretic types of rotavirus RNA during a 4-yr study of gastroenteritis in Tucumán].

作者信息

Komaid J A, de Caillou S L, Suárez A M, de Castagnaro N R

机构信息

Sección Virología, Facultad de Bioquímica, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina.

出版信息

Rev Argent Microbiol. 1990 Jul-Sep;22(3):123-9.

PMID:1966305

Abstract

Between May 1986 and November 1989 a total of 796 faecal samples obtained from children with symptoms of acute diarrhea were analyzed. Rotavirus (RV) was detected in 166 cases by polyacrylamide gel electrophoresis of the viral RNA (PAGE) (Fig. 1). This technique allowed us to identify 19 different electropherotypes (EPT) eleven with a long pattern and 8 with a short one (Fig. 2). Two EPT's were dominant during this study; one detected in 1986 only and the other one in 1987, 1988 and 1989. During the last months of 1989 a new EPT was detected. The 8 EPT's with short patterns were the causative organisms of an outbreak during the cold season of 1987 (Table 2). These results show that the rotaviral infection is endemic in the province of Tucumán, as well as its prevalence during the winter season (Table 1).

摘要

1986年5月至1989年11月期间，对796份来自患有急性腹泻症状儿童的粪便样本进行了分析。通过病毒RNA的聚丙烯酰胺凝胶电泳（PAGE）在166例中检测到轮状病毒（RV）（图1）。这项技术使我们能够识别出19种不同的电泳型（EPT），其中11种为长型，8种为短型（图2）。在本研究期间，有两种EPT占主导地位；一种仅在1986年检测到，另一种在1987年、1988年和1989年检测到。在1989年的最后几个月，检测到一种新的EPT。8种短型EPT是1987年寒冷季节一次暴发的病原体（表2）。这些结果表明，轮状病毒感染在图库曼省呈地方性流行，并且在冬季也有较高的流行率（表1）。