Okada Y, Suzuki K, Komuro K, Mizuochi T
Department of Blood Products, National Institute of Health, Tokyo, Japan.
J Virol. 1992 Sep;66(9):5177-82. doi: 10.1128/JVI.66.9.5177-5182.1992.
Maternal transmission of a murine leukemia virus (MuLV) mixture named LP-BM5 MuLV, which is knwon to induce murine AIDS (MAIDS), was investigated. Adult female C57BL/10 mice were inoculated intraperitoneally with LP-BM5 MuLV. When the virus-inoculated female mice developed splenomegaly or lymphadenopathy, they were mated with normal C57BL/10 male mice. Of 56 offspring born to MAIDS mothers, 14 appeared to develop MAIDS, as assessed by the occurrence of splenomegaly or lymphadenopathy as well as the mitogen response of spleen cells. The occurrence of MAIDS in offspring was found to be accompanied by the maternal transmission and expansion of a defective virus genome from which almost the entire pol and env regions are deleted. On the other hand, the ecotropic helper virus genome was detected in all offspring regardless of the occurrence of MAIDS. To examine the mode of maternal transmission of LP-BM5 MuLV, foster-nursing experiments were conducted. The ecotropic helper viruses were found in all normal offspring nursed by a MAIDS mother, and some of them developed MAIDS. In contrast, none of offspring born to a MAIDS mother that were nursed by an uninfected foster mother either carried the LP-BM5 MuLV or developed MAIDS. Finally, both the defective and the ecotropic helper viruses were detected in LP-BM5 MuLV-infected mother's milk. These results indicated that maternal transmission of LP-BM5 MuLV occurs with a high frequency and is mediated by mother's milk.
对一种名为LP - BM5 MuLV的鼠白血病病毒混合物的母体传播进行了研究，已知该病毒可诱发鼠类艾滋病（MAIDS）。成年雌性C57BL/10小鼠经腹腔接种LP - BM5 MuLV。当接种病毒的雌性小鼠出现脾肿大或淋巴结病时，将它们与正常的C57BL/10雄性小鼠交配。在MAIDS母亲所生的56只后代中，有14只似乎患上了MAIDS，这是通过脾肿大或淋巴结病的出现以及脾细胞的丝裂原反应来评估的。发现后代中MAIDS的发生伴随着一种缺陷病毒基因组的母体传播和扩增，该基因组几乎删除了整个pol和env区域。另一方面，无论是否发生MAIDS，在所有后代中都检测到了嗜亲性辅助病毒基因组。为了研究LP - BM5 MuLV的母体传播方式，进行了代乳实验。在由MAIDS母亲哺育的所有正常后代中都发现了嗜亲性辅助病毒，其中一些后代患上了MAIDS。相比之下，由未感染的代孕母亲哺育的MAIDS母亲所生的后代中，没有一只携带LP - BM5 MuLV或患上MAIDS。最后，在LP - BM5 MuLV感染的母亲的乳汁中检测到了缺陷病毒和嗜亲性辅助病毒。这些结果表明，LP - BM5 MuLV的母体传播频率很高，并且是由母乳介导的。
