Walz C, Schlehofer J R, Flentje M, Rudat V, zur Hausen H
Forschungsschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
J Virol. 1992 Sep;66(9):5651-7. doi: 10.1128/JVI.66.9.5651-5657.1992.
Infection with the helper virus-dependent human parvovirus adeno-associated virus (AAV) is known to interfere with cellular transformation in vitro and oncogenesis in vivo. Here we report on sensitization to gamma irradiation by AAV infection of cells in culture and of tumors established from HeLa cells grafted into immunodeficient (nude) mice: infection of HeLa cells with AAV type 2 enhanced cell killing and reduced plating efficiency after irradiation compared with uninfected cells. Similarly, HeLa cell tumors in nude mice displayed a reduced growth rate and were more sensitive to gamma irradiation when the animals were infected with AAV type 2 prior to or after tumor cell inoculation. Since no pathogenicity is known for AAV, the ability of this virus to render radiotherapy of human tumor cells more efficient may up open novel approaches in cancer treatment.
已知依赖辅助病毒的人细小病毒腺相关病毒（AAV）感染可在体外干扰细胞转化并在体内干扰肿瘤发生。在此，我们报告了在培养细胞以及将HeLa细胞移植到免疫缺陷（裸）小鼠中形成的肿瘤中，AAV感染可使细胞对γ射线致敏：与未感染的细胞相比，用2型AAV感染HeLa细胞可增强照射后的细胞杀伤作用并降低平板接种效率。同样，当在肿瘤细胞接种之前或之后用2型AAV感染动物时，裸鼠中的HeLa细胞瘤生长速率降低且对γ射线更敏感。由于AAV尚无致病性，这种病毒使人类肿瘤细胞放疗更有效的能力可能会为癌症治疗开辟新途径。
