Boshart M, Gissmann L, Ikenberg H, Kleinheinz A, Scheurlen W, zur Hausen H
EMBO J. 1984 May;3(5):1151-7. doi: 10.1002/j.1460-2075.1984.tb01944.x.
DNA of a new papillomavirus type was cloned from a cervical carcinoma biopsy. Two EcoRI clones of 7.8 and 6.9 kb in length were obtained, the latter contained a 900-bp deletion. The BamHI fragments of both clones were used to characterize the DNA. It represents a distinct type of papillomavirus as determined by its size, its cross-hybridization with DNA of other papillomavirus types under conditions of low stringency only, the co-linear alignment of its genome with HPV 6 and HPV 16 prototypes and its occasional occurrence as oligomeric episomes. We tentatively propose to designate it as HPV 18. DNA hybridizing with HPV 18 under stringent conditions was detected in 9/36 cervical carcinomas from Africa and Brazil, in 2/13 cervical tumors from Germany and 1/10 penile carcinomas. Benign tumors (17 cervical dysplasias, 29 genital warts), eight carcinomata in situ and 15 biopsies of normal cervical tissue were devoid of detectable HPV 18 DNA. HPV 18-related DNA was found, however, in cells of the HeLa, KB and C4-1 lines all derived from cervical cancer. The state of the viral DNA was investigated in four cervical cancer biopsies. The data reveal that the DNA might be integrated into the host cell genome. One tumor provided evidence for head to tail tandem repeats some of which persisted as circular episomes.
从一例宫颈癌活检组织中克隆出一种新型乳头瘤病毒的DNA。获得了两个长度分别为7.8kb和6.9kb的EcoRI克隆，后者含有一个900bp的缺失。两个克隆的BamHI片段用于对该DNA进行特征分析。根据其大小、仅在低严谨条件下与其他乳头瘤病毒类型的DNA交叉杂交、其基因组与HPV 6和HPV 16原型的共线性排列以及其偶尔以寡聚游离基因形式出现，它代表了一种独特类型的乳头瘤病毒。我们初步提议将其命名为HPV 18。在来自非洲和巴西的36例宫颈癌中的9例、来自德国的13例宫颈肿瘤中的2例以及10例阴茎癌中的1例中，检测到在严谨条件下与HPV 18杂交的DNA。良性肿瘤（17例宫颈发育异常、29例尖锐湿疣）、8例原位癌和15例正常宫颈组织活检均未检测到可检测到的HPV 18 DNA。然而，在所有源自宫颈癌的HeLa、KB和C4-1细胞系中发现了与HPV 18相关的DNA。对4例宫颈癌活检组织中的病毒DNA状态进行了研究。数据显示该DNA可能已整合到宿主细胞基因组中。一个肿瘤提供了头对头串联重复的证据，其中一些以环状游离基因形式持续存在。
