Hjerpe A, Bistoletti P, Dillner L, Mårdh P A, Magnusson G
Department of Pathology, Karolinska Institute, Huddinge University Hospital, Sweden.
Microbiologica. 1992 Jul;15(3):297-301.
A technique for the detection and typing of genital infections with human papilloma virus (HPV) is described. Following a non-invasive sampling and a simplified preparation procedure, the analysis was performed as a combined dot-blot and Southern blot analysis, where the former test was used to exclude cases without demonstrable content of HPV DNA. The subgenomic probes used in the Southern blot analyses have a higher specificity than can be achieved with genomic ones, and only one band is obtained for each of HPV types 6, 11, 16, 18, 31, 33 and 35. Extra bands indicating the presence of cross-reacting HPV of undetermined type occurred in only 0.7% of the 1,268 cases tested. HPV was demonstrated in 7.8% of the 612 health control samples and in 16% of 634 consecutive samples from patients with symptoms. All but type 18 occurred more commonly in symptomatic than in asymptomatic patients. Presence of HPV also correlated to cervical intraepithelial neoplasia (CIN) in simultaneously collected smears. The HPV was demonstrated in 13% of patients with normal cytology, in 45% with CIN and in 82% of patients with invasive carcinoma. All types of HPV occurred more commonly in CIN patients, while only types 16, 18, 31 and 33 were found in the patients with cancer.
本文描述了一种检测人乳头瘤病毒（HPV）生殖器感染及分型的技术。经过无创采样和简化的样本制备程序后，采用斑点杂交和Southern杂交相结合的方法进行分析，其中前者用于排除HPV DNA含量无法检测到的病例。Southern杂交分析中使用的亚基因组探针比基因组探针具有更高的特异性，对于HPV 6、11、16、18、31、33和35型，每种类型仅获得一条条带。在1268例检测病例中，仅0.7%出现额外条带，提示存在未定型的交叉反应性HPV。在612份健康对照样本中，7.8%检测到HPV；在634份有症状患者的连续样本中，16%检测到HPV。除18型外，所有其他类型在有症状患者中比无症状患者中更常见。HPV的存在也与同时采集涂片的宫颈上皮内瘤变（CIN）相关。在细胞学正常的患者中，13%检测到HPV；CIN患者中，45%检测到HPV；浸润癌患者中，82%检测到HPV。所有类型的HPV在CIN患者中更常见，而在癌症患者中仅发现16、18、31和33型。
