Migliarini A M, Terzaghi M L, Melemeni D, Gilardi E, Remotti G
Cattedra di Patologia Ostetrico-Ginecologica, Università di Milano.
Ann Ostet Ginecol Med Perinat. 1991 May-Jun;112(3):135-45.
One-hundred ten cervical or vaginal bioptic samples have been submitted to in-situ HPV typing with biotinylated probes. All samples had been taken from women with colposcopic findings suggesting HPV infection, and at histopathology showed koilocytosis sometimes associated with CIN or VAIN. Sixty-four samples were positive (16 for HPV 6/11 - 37 for HPV 16/18, 11 for HPV 31/35/51). Histopathological and cytological specimens were reviewed to detect the possible existence of morphological patterns typical of the various viral types. Papillomatosis, slightly increased nuclear size with finely granular chromatin, low number of mitoses, and scarce cyto-polymorphism, were associated with HPV 6/11, as well as the absence of intraepithelial neoplasia. A higher number of mitoses (and/or atypical ones), cyto-polymorphism, association with intraepithelial neoplasia, nuclear vacuolization, nuclei of increased size, with irregular borders, hyperchromic, with dark and lumpy chromatin were indicative of high or medium risk HPV (16/18 or 31/35/51). In cytological smears also the finding of dyskeratocytes was associated with these viral types, while multiple nuclei were present in the case of HPV 31/35/51 detection.
110份宫颈或阴道活检样本已使用生物素化探针进行原位HPV分型。所有样本均取自阴道镜检查结果提示HPV感染的女性，组织病理学检查显示有时伴有CIN或VAIN的挖空细胞。64份样本呈阳性（16份为HPV 6/11，37份为HPV 16/18，11份为HPV 31/35/51）。对组织病理学和细胞学标本进行复查，以检测各种病毒类型典型形态学模式的可能存在情况。乳头瘤病、核大小略有增加且染色质呈细颗粒状、有丝分裂数量少以及细胞多形性稀少，与HPV 6/11相关，且无上皮内瘤变。有丝分裂数量较多（和/或非典型有丝分裂）、细胞多形性、与上皮内瘤变相关、核空泡化、核大小增加、边界不规则、染色质深染且呈块状，提示高危或中危HPV（16/18或31/35/51）。在细胞学涂片中，角化不良细胞的发现也与这些病毒类型相关，而在检测到HPV 31/35/51时会出现多核现象。
