血友病患者在接受α-2B干扰素治疗期间血浆中丙型肝炎病毒RNA的消失情况。

Disappearance of hepatitis C virus RNA in plasma during interferon alpha-2B treatment in hemophilia patients.

作者信息

Bresters D, Mauser-Bunschoten E P, Cuypers H T, Lelie P N, Han J H, Jansen P L, Houghton M, Reesink H W

机构信息

Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.

出版信息

Scand J Gastroenterol. 1992;27(3):166-8. doi: 10.3109/00365529208999943.

DOI:10.3109/00365529208999943

PMID:1323873

Abstract

To establish the effect of interferon alpha-2B (IFN-alpha) treatment on hepatitis C virus (HCV) viremia, rather than monitor the alanine aminotransferase (ALAT) values we measured HCV-RNA by cDNA-polymerase chain reaction (cDNA-PCR) in plasma before and during IFN-alpha treatment. Eight hemophilia patients with chronic hepatitis C were treated with IFN-alpha for 24 weeks: 5 MU daily for 2 weeks, 2.5 MU daily for 4 weeks, and 1.5 MU three times a week for 18 weeks. HCV-RNA, as measured by cDNA-PCR, was present in all patients before treatment. After 24 weeks of treatment HCV-RNA was no longer detectable in three of eight (37.5%) patients, whereas only one of eight (12.5%) patients showed complete ALAT normalization. In three of eight patients a transient response to IFN-alpha was seen, with renewed HCV-RNA detection after dose reduction. HCV-RNA measurement by cDNA-PCR appeared to be more sensitive in detecting relapse than ALAT measurement.

摘要

为确定α-2B干扰素（IFN-α）治疗对丙型肝炎病毒（HCV）血症的影响，我们在IFN-α治疗前及治疗期间通过血浆中的cDNA聚合酶链反应（cDNA-PCR）检测HCV-RNA，而不是监测丙氨酸转氨酶（ALAT）值。8例慢性丙型肝炎血友病患者接受IFN-α治疗24周：前2周每天5 MU，接下来4周每天2.5 MU，最后18周每周三次每次1.5 MU。治疗前所有患者经cDNA-PCR检测均存在HCV-RNA。治疗24周后，8例患者中有3例（37.5%）不再能检测到HCV-RNA，而8例患者中只有1例（12.5%）的ALAT完全恢复正常。8例患者中有3例出现对IFN-α的短暂反应，在剂量减少后再次检测到HCV-RNA。通过cDNA-PCR检测HCV-RNA在检测复发方面似乎比检测ALAT更敏感。