McPhail D B, Sibbald A M
Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen, Scotland.
Free Radic Res Commun. 1992;16(5):277-84. doi: 10.3109/10715769209049180.
The formation of reactive free radical species in sheep erythrocytes challenged with dimethyldisulphide, a brassica-derived haemolysin, has been investigated by electron spin resonance spectroscopy using the spin trap alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone. Erythrocytes exposed to this agent undergo a burst of free radical activity as demonstrated by the appearance of a spin adduct. The results suggest that haemolytic anaemia which can occur in sheep grazing forage brassicas is a consequence of oxidative stress.
使用自旋捕捉剂α-(4-吡啶基1-氧化物)-N-叔丁基硝酮，通过电子自旋共振光谱法研究了用二甲基二硫（一种源自芸苔属植物的溶血素）攻击的绵羊红细胞中活性自由基的形成。暴露于该试剂的红细胞会出现自旋加合物，表明发生了自由基活性爆发。结果表明，放牧芸苔属牧草的绵羊中可能出现的溶血性贫血是氧化应激的结果。
