通过对贝伦斯技术进行改进，对从喂食和禁食大鼠肝脏中分离出的细胞核中的两种碱性磷酸酶进行研究。
Studies of two types of alkaline phosphatase in nuclei isolated from the livers of fed and fasted rats by a modification of the Behrens technique.
作者信息
EMERY A J, DOUNCE A L
出版信息
J Biophys Biochem Cytol. 1955 Jul 25;1(4):331-8. doi: 10.1083/jcb.1.4.331.
Abstract
- Rat liver nuclei were isolated from normal rats and rats fasted for 36 hours by a slight modification of the Behrens technique. 2. The nucleus of the rat liver cell contains two types of alkaline phosphatase. This confirms the previous findings on rat liver nuclei isolated in aqueous media. 3. The one type of alkaline phosphatase is not activated by magnesium ions, and this enzyme is very strongly bound to structural material of the nucleus. The other type of alkaline phosphatase is activated by magnesium ions, and this enzyme is probably free to diffuse from cytoplasm to nucleus and vice versa through the nuclear membrane. 4. Fasting caused a pronounced decrease of protein in general and of the alkaline phosphatase which is activated by magnesium ions from the nucleus of the rat liver cell, while the alkaline phosphatase that is not activated by magnesium was less affected.
摘要
- 通过对贝伦斯技术稍作修改，从正常大鼠和禁食36小时的大鼠中分离出大鼠肝细胞核。2. 大鼠肝细胞的细胞核含有两种碱性磷酸酶。这证实了先前在水性介质中分离大鼠肝细胞核的研究结果。3. 一种碱性磷酸酶不受镁离子激活，这种酶与细胞核的结构物质紧密结合。另一种碱性磷酸酶受镁离子激活，这种酶可能通过核膜在细胞质和细胞核之间自由扩散。4. 禁食总体上导致蛋白质显著减少，以及大鼠肝细胞核中受镁离子激活的碱性磷酸酶减少，而不受镁激活的碱性磷酸酶受影响较小。
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