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在非水介质中分离细胞核。

The isolation of cell nuclei in non-aqueous media.

作者信息

ALLFREY V, STERN H, MIRSKY A E, SAETREN H

出版信息

J Gen Physiol. 1952 Jan;35(3):529-54. doi: 10.1085/jgp.35.3.529.

DOI:10.1085/jgp.35.3.529
PMID:14898034
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2147330/
Abstract
  1. A modified Behrens procedure is described for the isolation of nuclei from avian erythrocytes and from the liver, kidney, thymus, pancreas, heart, and intestinal mucosa of the calf or horse. 2. The purity of these nuclei has been established by staining reactions, enzyme studies, and immunological tests for serum proteins. 3. Evidence is presented to show that a transport of cytoplasmic proteins into the nucleus does not occur during the isolation. 4. Nuclei prepared in non-aqueous media contain considerably more protein and a very different enzyme composition from that observed in nuclei prepared by "homogenization" techniques in dilute citric acid. 5. The suitability of nuclei prepared in organic media for the study of intracellular enzyme distribution is discussed.
摘要
  1. 描述了一种改良的贝伦斯方法，用于从小鸡红细胞以及小牛或马的肝脏、肾脏、胸腺、胰腺、心脏和肠黏膜中分离细胞核。2. 通过染色反应、酶研究以及血清蛋白的免疫学检测确定了这些细胞核的纯度。3. 有证据表明，在分离过程中不会发生细胞质蛋白向细胞核的转运。4. 在非水介质中制备的细胞核所含蛋白质多得多，且酶组成与在稀柠檬酸中通过“匀浆”技术制备的细胞核非常不同。5. 讨论了在有机介质中制备的细胞核用于研究细胞内酶分布的适用性。

相似文献

1
The isolation of cell nuclei in non-aqueous media.在非水介质中分离细胞核。
J Gen Physiol. 1952 Jan;35(3):529-54. doi: 10.1085/jgp.35.3.529.
2
Soluble enzymes of nuclei isolated in sucrose and nonaqueous media; a comparative study.在蔗糖和非水介质中分离得到的细胞核可溶性酶：一项比较研究。
J Gen Physiol. 1953 Nov 20;37(2):177-87. doi: 10.1085/jgp.37.2.177.
3
Some enzymes of isolated nuclei.分离细胞核的一些酶。
J Gen Physiol. 1952 Jan;35(3):559-78. doi: 10.1085/jgp.35.3.559.
4
Some aspects of the desoxyribonuclease activities of animal tissues.动物组织中脱氧核糖核酸酶活性的某些方面。
J Gen Physiol. 1952 Nov;36(2):227-41. doi: 10.1085/jgp.36.2.227.
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The isolation of wheat germ nuclei and some aspects of their glycolytic metabolism.小麦胚细胞核的分离及其糖酵解代谢的某些方面。
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Uptake of glycine-N15 by components of cell nuclei.细胞核各组分对甘氨酸-N15的摄取。
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Free amino acids and nucleic acid content of cell nuclei isolated by a modification of Behrens' technique.通过改良贝伦斯技术分离得到的细胞核的游离氨基酸和核酸含量
J Gen Physiol. 1950 May 20;33(5):629-42. doi: 10.1085/jgp.33.5.629.
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METHODS FOR THE PURIFICATION OF THYMUS NUCLEI AND THEIR APPLICATION TO STUDIES OF NUCLEAR PROTEIN SYNTHESIS.胸腺细胞核的纯化方法及其在核蛋白合成研究中的应用。
J Cell Biol. 1964 May;21(2):213-31. doi: 10.1083/jcb.21.2.213.
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Studies of two types of alkaline phosphatase in nuclei isolated from the livers of fed and fasted rats by a modification of the Behrens technique.通过对贝伦斯技术进行改进，对从喂食和禁食大鼠肝脏中分离出的细胞核中的两种碱性磷酸酶进行研究。
J Biophys Biochem Cytol. 1955 Jul 25;1(4):331-8. doi: 10.1083/jcb.1.4.331.
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Mononucleotides of the cell nucleus.细胞核的单核苷酸
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引用本文的文献

1
Mechanochemistry of nucleosides, nucleotides and related materials.核苷、核苷酸及相关物质的机械化学
Beilstein J Org Chem. 2018 Apr 27;14:955-970. doi: 10.3762/bjoc.14.81. eCollection 2018.
2
Tissue fraction-past and present.组织分级——过去与现在。
J Cell Biol. 1971 Jul 1;50(1):20.
3
Diphosphopyridine nucleotide pyrophosphorylase in the nuclei isolated from poisoned and regenerating rat liver.从中毒和再生大鼠肝脏中分离出的细胞核中的二磷酸吡啶核苷酸焦磷酸化酶
Biochem J. 1961 Sep;80(3):481-7. doi: 10.1042/bj0800481.
4
Chloroplast Isolation in Nonaqueous Media.非水介质中叶绿体的分离
Plant Physiol. 1959 Jan;34(1):56-61. doi: 10.1104/pp.34.1.56.
5
The Isolation of Nuclei and Oxidative Particles from Viable Wheat Embryos.从活的小麦胚中分离细胞核和氧化颗粒。
Plant Physiol. 1957 Mar;32(2):124-9. doi: 10.1104/pp.32.2.124.
6
The Physical Environment of Chloroplasts as Related to their Morphology and Activity in Vitro.叶绿体的物理环境与其体外形态和活性的关系
Plant Physiol. 1954 Sep;29(5):448-58. doi: 10.1104/pp.29.5.448.
7
AMINO ACID ACTIVATION AND TRANSFER TO RIBONUCLEIC ACIDS IN THE CELL NUCLEUS.细胞核中氨基酸的活化及向核糖核酸的转移
Proc Natl Acad Sci U S A. 1959 Oct;45(10):1461-70. doi: 10.1073/pnas.45.10.1461.
8
AMINO ACID INCORPORATION BY ISOLATED THYMUS NUCLEI. I. THE ROLE OF DESOXYRIBONUCLEIC ACID IN PROTEIN SYNTHESIS.离体胸腺细胞核的氨基酸掺入。I. 脱氧核糖核酸在蛋白质合成中的作用。
Proc Natl Acad Sci U S A. 1954 Oct;40(10):881-5. doi: 10.1073/pnas.40.10.881.
9
The incorporation of N15 glycine by avian erythrocytes and reticulocytes in vitro.鸡红细胞和网织红细胞在体外对N15甘氨酸的摄取。
J Gen Physiol. 1952 Jul;35(6):841-6. doi: 10.1085/jgp.35.6.841.
10
Some enzymes of isolated nuclei.分离细胞核的一些酶。
J Gen Physiol. 1952 Jan;35(3):559-78. doi: 10.1085/jgp.35.3.559.

本文引用的文献

1
The Nucleoprotein Content of Whole Nuclei.完整细胞核的核蛋白含量
Proc Natl Acad Sci U S A. 1949 Jan;35(1):66-71. doi: 10.1073/pnas.35.1.66.
2
Free amino acids and nucleic acid content of cell nuclei isolated by a modification of Behrens' technique.通过改良贝伦斯技术分离得到的细胞核的游离氨基酸和核酸含量
J Gen Physiol. 1950 May 20;33(5):629-42. doi: 10.1085/jgp.33.5.629.
3
Some enzymes of isolated nuclei.分离细胞核的一些酶。
J Gen Physiol. 1952 Jan;35(3):559-78. doi: 10.1085/jgp.35.3.559.
4
Cytochemical studies of mammalian tissues; the isolation of cell components by differential centrifugation: a review.哺乳动物组织的细胞化学研究；通过差速离心法分离细胞成分：综述。
Cancer Res. 1951 Jan;11(1):1-22.