在非水介质中分离细胞核。
The isolation of cell nuclei in non-aqueous media.
作者信息
ALLFREY V, STERN H, MIRSKY A E, SAETREN H
出版信息
J Gen Physiol. 1952 Jan;35(3):529-54. doi: 10.1085/jgp.35.3.529.
Abstract
- A modified Behrens procedure is described for the isolation of nuclei from avian erythrocytes and from the liver, kidney, thymus, pancreas, heart, and intestinal mucosa of the calf or horse. 2. The purity of these nuclei has been established by staining reactions, enzyme studies, and immunological tests for serum proteins. 3. Evidence is presented to show that a transport of cytoplasmic proteins into the nucleus does not occur during the isolation. 4. Nuclei prepared in non-aqueous media contain considerably more protein and a very different enzyme composition from that observed in nuclei prepared by "homogenization" techniques in dilute citric acid. 5. The suitability of nuclei prepared in organic media for the study of intracellular enzyme distribution is discussed.
摘要
- 描述了一种改良的贝伦斯方法，用于从小鸡红细胞以及小牛或马的肝脏、肾脏、胸腺、胰腺、心脏和肠黏膜中分离细胞核。2. 通过染色反应、酶研究以及血清蛋白的免疫学检测确定了这些细胞核的纯度。3. 有证据表明，在分离过程中不会发生细胞质蛋白向细胞核的转运。4. 在非水介质中制备的细胞核所含蛋白质多得多，且酶组成与在稀柠檬酸中通过“匀浆”技术制备的细胞核非常不同。5. 讨论了在有机介质中制备的细胞核用于研究细胞内酶分布的适用性。
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本文引用的文献
1
The Nucleoprotein Content of Whole Nuclei.完整细胞核的核蛋白含量
Proc Natl Acad Sci U S A. 1949 Jan;35(1):66-71. doi: 10.1073/pnas.35.1.66.
2
Free amino acids and nucleic acid content of cell nuclei isolated by a modification of Behrens' technique.通过改良贝伦斯技术分离得到的细胞核的游离氨基酸和核酸含量
J Gen Physiol. 1950 May 20;33(5):629-42. doi: 10.1085/jgp.33.5.629.
3
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J Gen Physiol. 1952 Jan;35(3):559-78. doi: 10.1085/jgp.35.3.559.
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