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粗糙脉孢菌分生孢子萌发因子的分离与鉴定。

Isolation and identification of the conidial germination factor of Neurospora crassa.

作者信息

Horowitz N H, Charlang G, Horn G, Williams N P

出版信息

J Bacteriol. 1976 Jul;127(1):135-40. doi: 10.1128/jb.127.1.135-140.1976.

Abstract

The germination-essential substance (germination factor [GF]) that is lost from conidia of Neurospora crassa on exposure to solutions of low water activity has been isolated and identified as a group of iron-transport compounds, or siderochromes. The principal siderochrome of conidia is ferricrocin, a cyclic hexapeptide. A closely related substance, ferrichrome C, is tentatively identified as a minor constituent. The same substances are also present in extracts of mycelium along with small amounts of a third siderochrome, which has not been identified. The GF activity of culture filtrates is due to coprogen, the only siderochrome previously identified with N. crassa.

摘要

粗糙脉孢菌分生孢子在暴露于低水分活度溶液时会失去的萌发必需物质(萌发因子[GF])已被分离并鉴定为一组铁转运化合物,即铁色素。分生孢子的主要铁色素是铁载体蛋白,一种环状六肽。一种密切相关的物质,铁色素C,被初步鉴定为次要成分。同样的物质也存在于菌丝体提取物中,同时还有少量尚未鉴定的第三种铁色素。培养滤液的GF活性归因于粪生粪壳菌素,这是之前唯一鉴定出与粗糙脉孢菌有关的铁色素。

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本文引用的文献

1
Germination and growth of neurospora at low water activities.低水分活度下脉孢菌的萌发与生长
Proc Natl Acad Sci U S A. 1971 Feb;68(2):260-2. doi: 10.1073/pnas.68.2.260.
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Studies on iron metabolism in Neurospora crassa.粗糙脉孢菌中铁代谢的研究。
Arch Biochem Biophys. 1965 Jul;111(1):147-52. doi: 10.1016/0003-9861(65)90333-4.
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Folin-Ciocalteu reagent for the estimation of siderochromes.
Anal Biochem. 1965 Jul;12(1):106-12. doi: 10.1016/0003-2697(65)90147-8.
8
Hydroxamic acids of natural origin.天然来源的异羟肟酸。
Adv Enzymol Relat Areas Mol Biol. 1971;35:135-85. doi: 10.1002/9780470122808.ch4.

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