Bates W K, Wilson J F
J Bacteriol. 1974 Feb;117(2):560-7. doi: 10.1128/jb.117.2.560-567.1974.
In nutrient medium containing 3.22 M ethylene glycol or glycerol, conidia of Neurospora crassa grow as single cells, without forming the germ tubes characteristic of normal morphological germination. Ethylene glycol is more effective than glycerol in producing this response. After growth in ethylene glycol medium for a suitable time, the cells are easily disrupted by an abrupt decrease in osmotic pressure. Osmotic disruption yields intact nuclei and mitochondria, although mitochondrial fractions obtained in this way show significantly reduced concentrations of cytochromes c + c(1), as compared to those observed for comparable fractions obtained from vegetative hyphae. Cell cultures gradually adapted to lower concentrations of the glycol show a much higher degree of synchrony in the formation of germ tubes than do untreated conidia.
在含有3.22 M乙二醇或甘油的营养培养基中，粗糙脉孢菌的分生孢子以单细胞形式生长，不形成正常形态萌发所特有的芽管。在产生这种反应方面，乙二醇比甘油更有效。在乙二醇培养基中生长适当时间后，细胞很容易因渗透压突然降低而破裂。渗透破裂产生完整的细胞核和线粒体，尽管以这种方式获得的线粒体组分与从营养菌丝获得的可比组分相比，细胞色素c + c(1)的浓度显著降低。与未处理的分生孢子相比，逐渐适应较低浓度乙二醇的细胞培养物在芽管形成方面表现出更高程度的同步性。
