Butler J R, Coukell M B
Department of Biology, York University, Ont.
Biochem Cell Biol. 1992 Feb;70(2):169-73. doi: 10.1139/o92-025.
When vegetative and early slug stage amoebae of Dictyostelium discoideum or Polysphondylium violaceum were lysed by filter breakage in a nuclear isolation buffer not containing detergents, substantial levels of a cGMP-binding activity with slow-dissociation kinetics were detected. After fractionation by centrifugation, 50% or more of this binding activity was associated with isolated nuclei. In addition, with Polysphondylium cells, the fraction of stable, nuclear-associated binding activity appeared to increase during cell aggregation. These results support the idea that cGMP might function in the nucleus during early development.
当盘基网柄菌或紫多头绒泡菌的营养期和早期蛞蝓体期变形虫在不含去污剂的核分离缓冲液中通过过滤破碎进行裂解时，检测到了具有缓慢解离动力学的大量cGMP结合活性。通过离心分级分离后，这种结合活性的50%或更多与分离出的细胞核相关。此外，对于紫多头绒泡菌细胞，在细胞聚集过程中，稳定的、与细胞核相关的结合活性部分似乎增加。这些结果支持了cGMP可能在早期发育过程中在细胞核中发挥作用的观点。
