Pan P, Wedner H J
Differentiation. 1979;14(1-2):113-8. doi: 10.1111/j.1432-0436.1979.tb01019.x.
Using indirect immunofluorescence technique, it has been possible to localize cyclic GMP in Polysphondylium violaceum cells. The bound cyclic nucleotide is localized throughout the cell during early stages, however, this staining increases and there is marked localization of cyclic GMP in the nuclear areas of the cells when aggregation is in full swing. Over 90% of the cells exhibited intense nuclear staining by 6 h and this decreased to less than 10% by 10 h.
利用间接免疫荧光技术，已能够在紫多头绒泡菌细胞中定位环磷酸鸟苷。在早期阶段，结合的环核苷酸在整个细胞中都有定位，然而，当聚集全面展开时，这种染色增加，并且环磷酸鸟苷在细胞的核区域有明显的定位。到6小时时，超过90%的细胞呈现强烈的核染色，到10小时时，这一比例降至不到10%。
