Nozaki S, Shimomura I, Funahashi T, Menju M, Kubo M, Matsuzawa Y
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Biochim Biophys Acta. 1992 Aug 19;1127(3):298-302. doi: 10.1016/0005-2760(92)90235-n.
We have studied the effects of triiodothyronine (T3) on the production of hepatic triacylglycerol lipase (HTGL) in the human hepatocellular carcinoma cell line, HepG2, by measuring its activity and mRNA levels. The HTGL activity released into the medium by heparin, increased after the addition of T3 in a both time- (27% increase after 24 and 75% increase after 48 h) and dose-dependent manner (maximum activity with over 0.2 micrograms/ml of T3 in the medium). Messenger RNA levels of HTGL in cells incubated with T3 for 24 and 48 h were increased by 33% and 98% compared to those of the control. These results suggest that the production of HTGL may be regulated by thyroid hormone at the level of gene expression.
我们通过测量人肝癌细胞系HepG2中肝三酰甘油脂肪酶（HTGL）的活性和mRNA水平，研究了三碘甲状腺原氨酸（T3）对其产生的影响。肝素释放到培养基中的HTGL活性，在添加T3后呈时间依赖性（24小时后增加27%，48小时后增加75%）和剂量依赖性（培养基中T3超过0.2微克/毫升时活性最高）增加。与对照组相比，T3孵育24小时和48小时的细胞中HTGL的信使RNA水平分别增加了33%和98%。这些结果表明，HTGL的产生可能在基因表达水平受到甲状腺激素的调节。
