枯草芽孢杆菌噬菌体SPO1的DNA聚合酶编码基因。
The DNA polymerase-encoding gene of Bacillus subtilis bacteriophage SPO1.
作者信息
Scarlato V, Gargano S
机构信息
International Institute of Genetics and Biophysics, Naples, Italy.
出版信息
Gene. 1992 Sep 1;118(1):109-13. doi: 10.1016/0378-1119(92)90256-o.
The bacteriophage SPO1 DNA polymerase-encoding gene, which contains a self-splicing intron, has been sequenced and its amino acid (aa) sequence has been deduced. The aa sequence of SPO1 DNA polymerase shows a high degree of similarity with that of DNA polymerase I from Escherichia coli (Po1I). Alignment with the sequences of Po1I, and the phi 29 and SPO1 DNA polymerases indicate that the aa residues that have been implicated in 3'----5' exonuclease activities are conserved.
含有一个自我剪接内含子的噬菌体SPO1 DNA聚合酶编码基因已被测序，其氨基酸（aa）序列也已推导得出。SPO1 DNA聚合酶的氨基酸序列与大肠杆菌DNA聚合酶I（Po1I）的氨基酸序列高度相似。与Po1I、phi 29和SPO1 DNA聚合酶的序列比对表明，与3'→5'核酸外切酶活性相关的氨基酸残基是保守的。
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