Goodrich-Blair H, Shub D A
Department of Biological Sciences, University at Albany, SUNY 12222.
Nucleic Acids Res. 1994 Sep 11;22(18):3715-21. doi: 10.1093/nar/22.18.3715.
A previous report described the discovery of a group I, self-splicing intron in the DNA polymerase gene of the Bacillus subtilis bacteriophage SPO1 (1). In this study, the DNA polymerase genes of three close relatives of SPO1: SP82, 2C and phi e, were also found to be interrupted by an intron. All of these introns have group I secondary structures that are extremely similar to one another in primary sequence. Each is interrupted by an open reading frame (ORF) that, unlike the intron core or exon sequences, are highly diverged. Unlike the relatives of Escherichia coli bacteriophage T4, most of which do not have introns (2), this intron seems to be common among the relatives of SPO1.
先前的一份报告描述了在枯草芽孢杆菌噬菌体SPO1的DNA聚合酶基因中发现了一个I类自我剪接内含子（1）。在本研究中，还发现SPO1的三个近亲噬菌体：SP82、2C和phi e的DNA聚合酶基因也被一个内含子中断。所有这些内含子都具有I类二级结构，其一级序列彼此极为相似。每个内含子都被一个开放阅读框（ORF）中断，该开放阅读框与内含子核心或外显子序列不同，具有高度的差异性。与大肠杆菌噬菌体T4的亲属不同，其中大多数没有内含子（2），这种内含子似乎在SPO1的亲属中很常见。
