使用生物素化内皮素和抗生物素蛋白-琼脂糖对牛肺内皮素受体进行亲和色谱纯化。
Affinity chromatographic purification of bovine lung endothelin receptor using biotinylated endothelin and avidin-agarose.
作者信息
Hagiwara H, Nagasawa T, Lodhi K M, Kozuka M, Ito T, Hirose S
机构信息
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
出版信息
J Chromatogr. 1992 Apr 24;597(1-2):331-4. doi: 10.1016/0021-9673(92)80128-h.
Endothelin receptor was purified from bovine lung by affinity chromatography using biotinylated endothelin and avidin-agarose. Endothelin was biotinylated with sulphosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate, a reactive form of biotin with a cleavable spacer arm containing a disulphide bond designed for a simple elution by thiols. Starting from 3.5 kg of bovine lung, about 200 micrograms of pure receptor were obtained.
使用生物素化的内皮素和抗生物素蛋白-琼脂糖通过亲和色谱法从牛肺中纯化内皮素受体。内皮素用磺基琥珀酰亚胺基2-（生物素酰胺基）乙基-1,3-二硫代丙酸酯进行生物素化，这是一种具有可裂解间隔臂的生物素活性形式，该间隔臂含有二硫键，设计用于通过硫醇进行简单洗脱。从3.5千克牛肺开始，获得了约200微克的纯受体。
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