Hick S, Heidemann I, Soskic V, Müller-Esterl W, Godovac-Zimmermann J
Institut für Physiologische Chemie und Pathobiochemie, Johannes Gutenberg Universität, Mainz, Germany.
Eur J Biochem. 1995 Nov 15;234(1):251-7. doi: 10.1111/j.1432-1033.1995.251_c.x.
Bovine lung endothelin-B receptor has been isolated in good yield with a new procedure involving the use of endothelin-1 coupled to iminobiotin with a long spacer and avidin-agarose affinity chromatography. Contrary to previous reports, evidence has been obtained that the native form of this receptor corresponds to the full-length transcript expected on the basis of cDNA clones. The binding of endothelin to a variety of shortened fragments of the full receptor suggests that the long N-terminal sequence of this receptor has very little influence on the binding of endothelin and that the main determinants of the endothelin binding site might be constituted by residues in the sixth, and possibly the seventh, transmembrane helices.
通过一种新方法以高产率分离出了牛肺内皮素 - B 受体，该方法使用了与带有长间隔臂的亚氨基生物素偶联的内皮素 - 1 和抗生物素蛋白 - 琼脂糖亲和色谱法。与之前的报道相反，已获得证据表明该受体的天然形式与基于 cDNA 克隆预期的全长转录本相对应。内皮素与完整受体的各种缩短片段的结合表明，该受体的长 N 端序列对内皮素的结合影响很小，并且内皮素结合位点的主要决定因素可能由第六个，可能还有第七个跨膜螺旋中的残基构成。
