Traynor D, Kessin R H, Williams J G
Imperial Cancer Research Fund, Clare Hall Laboratory, Potters Bar, Herts, United Kingdom.
Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8303-7. doi: 10.1073/pnas.89.17.8303.
Dictyostelium transformants that overproduce the extracellular form of cyclic nucleotide phosphodiesterase and so accumulate a reduced amount of cAMP are blocked in development after aggregation in the form of a tight mound, prior to formation of the apical tip. In such mounds, prespore cell differentiation is repressed, and the apical accumulation of prestalk cells is greatly retarded. When a source of cAMP is placed below the arrested mounds, prestalk cells that would normally migrate in an apical direction instead sort downwards to the substratum. Thus, by acting as the chemoattractant that draws prestalk cells to the apex, cAMP signaling directs the formation of a patterned structure.
过量产生细胞外形式的环核苷酸磷酸二酯酶从而积累较少cAMP的盘基网柄菌转化体，在聚集后以紧密土堆的形式发育受阻，此时尚未形成顶端。在这样的土堆中，前孢子细胞分化受到抑制，前柄细胞的顶端积累大大延迟。当在停滞的土堆下方放置一个cAMP源时，通常会向顶端迁移的前柄细胞反而向下分选到底层。因此，通过作为将前柄细胞吸引到顶端的趋化因子，cAMP信号传导指导了一种模式化结构的形成。
