Han T, Takita H
J Surg Oncol. 1976;8(3):237-43. doi: 10.1002/jso.2930080309.
The inhibitory effect of the thoracic duct lymph of a patient with lung cancer on the "one-way" mixed lymphocyte reaction without cytoxicity is unequivocally demonstrated. The effect seems to be dose related. A moderate inhibition of mixed lymphocyte reaction is still observed, even if the responding cells are preincubated in the thoracic duct lymph for 1 hr only prior to the addition of stimulating cells. The inhibitory effect of thoracic duct lymph on the mixed lymphocyte reaction is no longer evident when the material is added 1-4 days after the beginning of culture. These observations suggest that the mechanism of the inhibitory effect of thoracic duct lymph may be a simple attachment of inhibitory factors to the receptor sites on the responding lymphocytes, causing interference in cell to cell interaction. The inhibitory effect of thoracic duct lymph collected 1 week after the thoracic duct drainage on mixed lymphocyte reaction is significantly lower than that of thoracic duct lymph collected at the beginning of the procedure. This indicates that the blocking effect of thoracic duct lymph can be easily removed by this technique; which is technically feasible in man. The interrelationship of the tumor-specific blocking factor, thoracic duct drainage, and tumor growth pattern are discussed with respect to the potential usefulness of this procedure as adjuvant immunotherapy in the management of patients with neoplastic diseases.
明确证实了肺癌患者胸导管淋巴液对无细胞毒性的“单向”混合淋巴细胞反应具有抑制作用。这种作用似乎与剂量相关。即使反应细胞仅在加入刺激细胞前于胸导管淋巴液中预孵育1小时，仍可观察到混合淋巴细胞反应受到中度抑制。当在培养开始1 - 4天后添加该物质时，胸导管淋巴液对混合淋巴细胞反应的抑制作用不再明显。这些观察结果表明，胸导管淋巴液抑制作用的机制可能是抑制因子简单地附着于反应淋巴细胞上的受体部位，从而干扰细胞间相互作用。胸导管引流1周后收集的胸导管淋巴液对混合淋巴细胞反应的抑制作用明显低于操作开始时收集的胸导管淋巴液。这表明通过该技术可轻易消除胸导管淋巴液的阻断作用；这在人体上技术上是可行的。就该操作作为肿瘤性疾病患者辅助免疫治疗的潜在用途而言，讨论了肿瘤特异性阻断因子、胸导管引流和肿瘤生长模式之间的相互关系。
