Isbister W H, Noonan F P, Halliday W J, Clunie G J
Cancer. 1975 May;35(5):1465-71. doi: 10.1002/1097-0142(197505)35:5<1465::aid-cncr2820350535>3.0.co;2-h.
Thoracic duct lymph was drained for 28 days from a patient with disseminated malignant melanoma. Lymphocytes were separated from the lymph by centrifugation, and returned to the patient daily. Biochemical and hematologic parameters were monitored in blood and lymph, and were maintained at satisfactory levels throughout the period. Cell-mediated immunity and specific blocking activity directed against melanoma antigens were examined by the leukocyte adherence inhibition test. Blocking factors in drained lymph fell to undetectable levels after 6 days' thoracic duct drainage, whereas it took 9 days for serum blocking factors to fall to similar levels. Peripheral blood leukocytes demonstrated cell-mediated immunity against melanoma antigens before and throughout the period of drainage, except for the immediate postoperative period. Within 24 hours of closure of the thoracic duct fistula, serum blocking activity had returned, and 17 days later the patient died.
对一名播散性恶性黑色素瘤患者的胸导管淋巴液进行了28天的引流。通过离心从淋巴液中分离出淋巴细胞，并每天回输给患者。监测血液和淋巴液中的生化和血液学参数，在整个期间这些参数均维持在满意水平。通过白细胞黏附抑制试验检测针对黑色素瘤抗原的细胞介导免疫和特异性阻断活性。胸导管引流6天后，引流淋巴液中的阻断因子降至检测不到的水平，而血清阻断因子降至类似水平则需要9天。除术后即刻外，引流前及引流期间外周血白细胞均表现出针对黑色素瘤抗原的细胞介导免疫。胸导管瘘关闭后24小时内，血清阻断活性恢复，17天后患者死亡。
