Devalia J L, Sapsford R J, Rusznak C, Davies R J
Department of Respiratory Medicine, St. Bartholomew's Hospital, London, United Kingdom.
Am J Respir Cell Mol Biol. 1992 Sep;7(3):270-7. doi: 10.1165/ajrcmb/7.3.270.
Although there is increasing evidence of a pathogenic role for eosinophils in the airway epithelium, there is little direct evidence which demonstrates that eosinophils influence epithelial cell activity in humans. We have cultured human nasal epithelial cells in vitro and studied the effect of isolated human eosinophils on the ciliary beat frequency (CBF) and cell membrane integrity of these cells after incubation in the absence or presence of 0.1 microM phorbol 12-myristate 13-acetate (PMA) or 0.1 mg/ml opsonized latex beads and the absence or presence of 10(-5) M nedocromil sodium. CBF was monitored by an analogue contrast-enhancement technique, and cell damage was assessed by release of 51Cr from the cells. Cell cultures were also assessed for the percentage of eosinophil cationic protein (ECP) released into the medium at the end of incubation. Neither 0.1 microM PMA, 0.1 mg/ml opsonized latex beads, 10(-5) M nedocromil sodium, nor eosinophils alone altered the CBF of the epithelial cells. PMA-stimulated eosinophils, however, attenuated the CBF significantly, from 10.2 +/- 0.3 to 8.8 +/- 0.4 Hz (P less than 0.05) after 15 h of incubation. Similarly, opsonized latex bead-stimulated eosinophils led to a significant attenuation of CBF from 9.2 +/- 0.3 to 8.4 +/- 0.3 Hz (P less than 0.05), 6.9 +/- 0.5 Hz (P less than 0.001), and 7.5 +/- 0.3 Hz (P less than 0.001) after 2, 15, and 24 h of incubation, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
尽管越来越多的证据表明嗜酸性粒细胞在气道上皮中具有致病作用，但几乎没有直接证据表明嗜酸性粒细胞会影响人类上皮细胞的活性。我们在体外培养了人鼻上皮细胞，并研究了分离出的人嗜酸性粒细胞在不存在或存在0.1微摩尔佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）或0.1毫克/毫升调理化乳胶珠以及不存在或存在10⁻⁵M奈多罗米钠的情况下孵育后，对这些细胞纤毛摆动频率（CBF）和细胞膜完整性的影响。通过模拟对比增强技术监测CBF，并通过细胞释放⁵¹Cr来评估细胞损伤。在孵育结束时，还评估了细胞培养物中释放到培养基中的嗜酸性粒细胞阳离子蛋白（ECP）的百分比。单独的0.1微摩尔PMA、0.1毫克/毫升调理化乳胶珠、10⁻⁵M奈多罗米钠或嗜酸性粒细胞均未改变上皮细胞的CBF。然而，PMA刺激的嗜酸性粒细胞在孵育15小时后显著降低了CBF，从10.2±0.3降至8.8±0.4赫兹（P＜0.05）。同样，调理化乳胶珠刺激的嗜酸性粒细胞在孵育2、15和24小时后分别导致CBF显著降低，从9.2±0.3降至8.4±0.3赫兹（P＜0.05）、6.9±0.5赫兹（P＜0.001）和7.5±0.3赫兹（P＜0.001）。（摘要截短于250字）
