Phillips M J, Mendis A H, Venaille T, Thompson P J, Robinson B W
Department of Respiratory Medicine, Queen Elizabeth II Medical Centre, Perth, Australia.
Drugs. 1989;37 Suppl 1:56-62; discussion 69-77. doi: 10.2165/00003495-198900371-00012.
A human amniotic epithelial membrane preparation was used as a model to study epithelial responses to neutrophils and eosinophils, obtained from normal subjects using Percoll density gradient methods, and activated by phorbyl myristate acetate (PMA). Incubation of activated neutrophils with the epithelial membrane resulted in epithelial cell desquamation, probably due to release of proteases. Activated eosinophils resulted in epithelial cell damage but no desquamation, an action that appeared to be mimicked by major basic protein (MBP). The addition of nedocromil sodium did not significantly inhibit neutrophil-induced epithelial cell desquamation. In one preliminary experiment, nedocromil sodium (10(-4) to 10(-5) mol/L) inhibited epithelial cell desquamation induced by a mixed population of eosinophils and neutrophils.
用人羊膜上皮膜制剂作为模型，研究上皮细胞对中性粒细胞和嗜酸性粒细胞的反应。中性粒细胞和嗜酸性粒细胞从正常受试者中通过Percoll密度梯度法获得，并用佛波醇肉豆蔻酸酯乙酸酯（PMA）激活。激活的中性粒细胞与上皮膜孵育导致上皮细胞脱落，这可能是由于蛋白酶的释放。激活的嗜酸性粒细胞导致上皮细胞损伤但无脱落，主要碱性蛋白（MBP）似乎能模拟这种作用。奈多罗米钠的添加并未显著抑制中性粒细胞诱导的上皮细胞脱落。在一项初步实验中，奈多罗米钠（10^(-4)至10^(-5)mol/L）抑制了嗜酸性粒细胞和中性粒细胞混合群体诱导的上皮细胞脱落。
